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Role of Secreted Aspartic Proteases in Candida Albicans Pathogenesis read online

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This dissertation, "Role of Secreted Aspartic Proteases in Candida Albicans Pathogenesis" by Haiping, Yang, 杨{274757}{275534}, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Assignment 3. 0 Hong Kong License. The captivate of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of type-setting and review of the dissertation. All freedoms not granted by the above license are retained by the author. Abstract: Candida. albicans is the key pathogen for oral candidiasis, and possesses comprehensive fury mechanisms to cause fungal flu in a emcee under proper predisposing settings. The secreted aspartic protease (Sap) family (which consists of 10 family extremities: Sap1-10) plays a critical role in the pathogenicity of Candida. Considering the increasing prevalence appraise of candidiasis global, understanding of Sap family's pathogenicity technique becomes critical for the maturity of any new antifungal proxies or remedial treatments. This mull over mainly investigated the virulent mechanisms of Sap7 and Sap9 in Candida flu, as well as the synergistic effect of VENEREAL disease protease inhibitors and fluconazole on antifungal treatment. The first quit of this mull over was to investigate the role of C. albicans SAP7 in fungal invasion to oral epithelial cells. sap7Δ/Δ mutant strain was created using a PCR-based gene disruption formula via homologous recombination. Compared the wild breed strain with sap7Δ/Δ mutant strain, the appears demonstrated that the deletion of SAP7 restrained C. albicans invasion to oral epithelial cells. C. albicans sap7Δ/Δ mutant strain significantly reduced the induced endocytosis by 47. 8%. Approximate 25% attenuation of sap7Δ/Δ mutant strain was internalized into the oral epithelial cells, and 27% reduction of the release of lactate dehydrogenase was detected. Opinion of reconstituted personal epithelial (RHE) meshes showed a significant reduction in the sum of attached and invaded fungal cells to oral epithelial meshes compared with the wild breed strain. The data implied that SAP7 contributes to C. albicans invasion to oral epithelial cells. The second quit of the mull over was to investigate the role of C. albicans SAP9 in hyphae construction and invasion skillfulness. Hyphal maturity was examined by both liquid and solid hyphal inducing publishings. Compared the sap9Δ/Δ mutant strain with wild breed strain, hyphal construction tests demonstrated that the yeast-to-hyphal semantic transition was skimpy in the sap9Δ/Δ mutant strain. Besides, quantitative turn the tables transcription-PCR (qRT-PCR) revealed a significant down arrangement of the interpretation of EFG1 (approximately 40%) in sap9Δ/Δ mutant strain. EFG1 is a transcription item gene positively mediating hyphae construction in C. albicans. It was also found that approximately 70% reduced sap9Δ/Δ mutant strain was endocytosed by emcee cells and 40% attenuation of emcee cell devastation caused by sap9Δ/Δ mutant strain, compared with the wild breed strains. The data strongly suggested that C. albicans Sap9 is a singular hyphal associated item via the cAMP-protein kinase A pathway, leading to invasion into the emcee cells and causing cell devastation. The final quit of the mull over was to investigate the synergistic antifungal effect of VENEREAL disease protease inhibitors and fluconazole on C. albicans. The appears showed that while the MIC of saquinavir and fluconazole required to suppress the candidal thickening was 700 μM and 32. 64μM respectively, the combination of saquinavir (12. 5 μM) and fluconazole (4. 08 μM) at lower bands could act synergistically against fungal thickening. SAP1-10 mRNA expressions are significantly less using combined treatment than fluconazole alone. Our data indicated that combined therapy might be an second way to reduce the dosage of antifungal drug and minimize multidrug resistance. DOI: 10. 5353/th_b5.

Role of Secreted Aspartic Proteases in Candida Albicans Pathogenesis read online or download

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  • Publisher: Open Dissertation Press
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  • Cover: Hardcover
  • Language: English
  • ISBN-10: 1361037881
  • ISBN-13: 978-1361037881
  • Dimensions: 8.5 x 0.6 x 11 inches
  • Weight: 1.8 pounds
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